Coding

Part:BBa_K896011:Design

Designed by: Shih-Hao Chen   Group: iGEM12_NYMU-Taipei   (2012-09-22)

Mnt (transporter, Cd2+ absorbing molecule)


Assembly Compatibility:
  • 10
    INCOMPATIBLE WITH RFC[10]
    Illegal XbaI site found at 1478
    Illegal SpeI site found at 1972
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal SpeI site found at 1972
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BamHI site found at 2519
  • 23
    INCOMPATIBLE WITH RFC[23]
    Illegal XbaI site found at 1478
    Illegal SpeI site found at 1972
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal XbaI site found at 1478
    Illegal SpeI site found at 1972
    Illegal AgeI site found at 67
  • 1000
    COMPATIBLE WITH RFC[1000]


Design Notes

C21.jpg

Source

Synechocystis sp. PCC 6803

References

  • ZHIQI HAO,1 SHAOLIN CHEN,1 AND DAVID B. WILSON1,2, Cloning, Expression, and Characterization of Cadmium and Manganese Uptake Genes from Lactobacillus plantarum, APPLIED AND ENVIRONMENTAL MICROBIOLOGY,Nov. 1999, p. 4746–4752 Vol. 65, No. 11